Transformações químicas realizadas em eremantolida C (1), uma lactona sesquiterpênica isolada de Lychnophora trichocarpha Spreng. originaram cinco novos derivados: 1′,2′-epoxieremantolida C (2), 5-n-propilamino-4,5-diidro-1′,2′-epoxieremantolida C (3), cloreto de 5-n-propilamônio-4,5-diidro-1′, 2′-epoxieremantolida C (4), cloreto de 5-n-propilamônio-4,5-diidroeremantolida C (5) e 16-O-etileremantolida C (6). As estruturas químicas de todos estes derivados foram elucidadas com base nos espectros de IV, EM, RMN de 1H e de 13C e por meio de técnicas 1D e 2D. Eremantolida C e os derivados 2, 4 e 5 foram avaliados frente a cepas tripomastigotas Y e CL de Trypanosoma cruzi. Eremantolida C inibiu completamente o crescimento de ambas as cepas de parasitas, enquanto todos os derivados foram parcialmente ativos contra a cepa CL e inativos contra a cepa Y.

Chemical transformations of eremantholide C (1), a sesquiterpene lactone that was isolated from Lychnophora trichocarpha Spreng. led to five new derivatives: 1′,2′- epoxyeremantholide C (2), 5-n-propylamine-4,5-dihydro-1′,2′-epoxyeremantholide C (3), 5-n-propylammonium-4,5-dihydro-1′,2′-epoxyeremantholide C chloride (4), 5-n-propylammonium-4,5-dihydroeremantolide C chloride (5) and 16-O-ethyleremantholide C (6). The structures of all these derivatives were assigned on the basis of IR, MS, 1H and 13C NMR data by 1D and 2D techniques. Eremantholide C and the derivatives 2, 4 and 5 were evaluated against trypomastigotes Y and CL strains of Trypanosoma cruzi. Eremantholide C completely inhibited the growth of both the parasites strains while all derivatives were partially active against the CL strain and inactive against the Y strain.

Species from genus Aspidosperma (Apocynaceae) are popularly employed to treat various diseases. This genus is characterized by the occurrence of indole alkaloids. Taking into account the various biological activities attributed to these alkaloids, the description of the chemical diversity in genus Aspidosperma is important. A review of simple carbolinic alkaloids isolated from species of various genera was published in 1979. In 1987, it was published another one dealing with the relationships between the chemical structures of the indole alkaloids and the evolution of Aspidosperma species. This work updates the information about the indole alkaloids isolated from Aspidosperma species.

O estudo fitoquímico das cascas do cerne de Aspidosperma nitidum permitiu o isolamento de um novo tipo de alcalóide indólico, contendo um sistema 1,2,9-triazabiciclo[7.2.1], que foi denominado braznitidumina 1. A caracterização de sua estrutura química foi realizada pela análises dos dados de IV, UV, ESI-EM e RMN de ¹H, 13C e 15N, empregando experimentos 1D e 2D (¹H ¹H COSY, ¹H ¹H NOESY, ¹H 13C HSQC e ¹H 13C HMBC). Pela análise do experimento ¹H ¹H NOESY, verificou-se que 1 apresenta uma conformação dobrada com aproximação entre os grupos indólico e imidazolidino-di-hidropirano. Essa configuração foi investigada por cálculos teóricos envolvendo otimizações de geometria (DFT/BLYP/6-31G*) para análise conformacional desse alcalóide, pela qual a distância entre os dois grupos mostrou-se compatível com as informações obtidas pelo experimento NOESY.

The phytochemical study of the stem bark of Aspidosperma nitidum led to the isolation of a new type of indole alkaloid with a 1,2,9-triazabicyclo[7.2.1] system, which has been called braznitidumine 1. The characterization of its chemical structure was carried out by IR, UV, ESIMS, and ¹H, 13C, and 15N NMR by using 1D and 2D (¹H ¹H COSY, ¹H ¹H NOESY, ¹H 13C HSQC and ¹H 13C HMBC) experiments. ¹H ¹H NOESY results showed that 1 presents a folded conformation with the approximation of the indole and the imidazolidine di-hydropyran groups. This configuration was investigated by theoretical calculations involving geometry optimization (DFT/BLYP/6-31G*) for the conformational analysis of this alkaloid. It confirmed the distance between the two groups in agreement with the NOESY experimental data.

Four known alkaloids, uleine, epiuleine, apparicine and desmethyluleine, besides the isoprenoids lupeol and stigmasterol were isolated from the stem bark of Aspidosperma parvifolium. Their identification was based on spectroscopic analysis (UV, IR, ¹H-NMR, 13C-NMR, MS). The chromatographic profile of the ethanolic extract was obtained by HPLC and uleine, epiuleine and apparicine were identified in the extract.

Embora pareça existir um "flavor" característico de mel, a grande variedade de flores disponíveis para a abelha, possibilita uma grande diversidade de flavor e aroma, indicando a presença de vários componentes voláteis. Alguns destes dependem da fisiologia da abelha, dos procedimentos após a colheita e no mel de melato há também a interferência de insetos sugadores e das formigas. Várias substâncias voláteis já foram identificadas, sendo algumas características de determinados méis uniflorais. Com o objetivo de encontrar uma substância volátil característica do mel de melato, seis amostras deste tipo de mel e seis amostras de mel floral foram analisadas usando extração por arraste de gás hidrogênio e cromatografia a gás acoplada a espectrometria de massas. Ácido acético foi encontrado em quatro amostras de mel de melato e em uma amostra de mel floral porém, com menor abundância.

Although honey seems to possess a characteristic flavor, variety of flowers avaiable to bees gives differents flavors and aromas, indicating the presence of various volatile compounds. Some of these also depends on the phisiology of the bee, the processing after harvest and in honeydew honey there is the interference of sucking insects and ants. Many volatile substances have already been identified and some of them are specific of typical unifloral honeys. With the purpose of finding a characteristic volatile substance of honeydew honey, six samples of this kind and six floral honeys were analysed by extraction using hidrogen steam and gas chromathography with mass spectrometry. Acetic acid has been found in four honeydew honey and in one floral honey, but with less abundance.

Four naphthoquinones, lapachol, a-lapachone, dehydro-a-lapachone and 4-hydroxy-a-lapachone were isolated from the ethanol extract of Zeyheria montana M. roots (Bignoniaceae) and were identified by spectroscopic methods. These naphthoquinones, except 4-hydroxy-a-lapachone, were quantified by HPLC, in the crude ethanol extracts of Z. montana roots. The separation of lapachol, a-lapachone and dehydro-a-lapachone was achieved and these compounds were identified in the chromatograms by their retention times and by their on line UV-spectra. The quantification of the three naphthoquinones, in four different samples showed medium contents of 11,0 mg of lapachol, 6,1 mg of a-lapachone and 4,3 mg of dehydro-a-lapachone, in 100 mg of Z.montana roots.